Nucleophosmin or NPM or nucleolar phospho-protein B23 is a type of protein that is found in humans which is encoded by the NPM1 gene. It is related to the nuclear ribonucleoprotein structures. It gets linked with nucleic acids, but it gets linked mostly with G-quadruplex that is formed in it. It is involved in the study of the biogenesis of ribosomes.
This protein may be of assistance to the small proteins during their transportation to the nucleus. It is a phosphor-protein basically found in the nucleus. It impacts several different cellular functions, like assembly and transport of ribosomes, regulation of DNA polymerase activity, etc. You can buy Anti-NPM (phospho-T234) NPM1 antibody kit to test its presence in the given sample.
It is located in the nucleus, but it can be transferred into the nucleoplasm in cases like serum starvation or in the treatment of anti-cancer medicines. NPM Antibody kit is used to detect endogenous levels of total NPM protein present in the given sample. Polyclonal antibodies are produced by making animals immune to a synthetic peptide which then corresponds to the residues around the human NPM.
These antibodies are then purified by protein A and by chromatography. NPM1 gene is regulated, mutated, and chromosomally transferred into many tumor types. Chromosomes with NPM1 present in them were found in patients suffering from lymphoma, acute leukemia, and myelodysplastic syndrome. It can be found in humans, rats, and mice.
Mice with NPM1present in them are prone to tumor development. In hard tumors, NPM1 is found repeatedly and it is believed that NPM1 could enhance the growth of the tumor by inactivating the tumor suppressor p53 path. But, on the flip side, at low levels, NPM1 could decrease the growth of tumors by the inhibiting duplication of centrosomes.